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cd133 cells  (Miltenyi Biotec)


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    Miltenyi Biotec cd133 cells
    Cd133 Cells, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 110 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd133+cells/CD133+MicroBead+Kit%2C+human+-+lyophilized/pmc12860264-131-7-14
    Average 95 stars, based on 110 article reviews
    cd133 cells - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Isolation:

    Article Title: Effects of Notch signaling on proliferation, angiogenesis, and adipogenesis of hemangioma-derived stem cells.
    Article Snippet: .. CD133+ cells were isolated from the single-cell suspension via magnetic-activated cell sorting (Miltenyi Biotec, Inc., Auburn, CA, USA) using anti-CD133 microbeads. .. Cells were cultured on fibronectin-coated plates (5 μg/cm2) in endothelial growth medium-2 (EGM-2; Lonza Group, Ltd., Basel, Switzerland) supplemented with 20% FBS and 1% penicillin/streptomycin (HyClone).

    Article Title: CXCL8/SDC1 axis mediates tumor stem cell interactions to drive remote transfer in thyroid cancer
    Article Snippet: Tohoku Hospital Pediatrics-1 (THP-1) cells (CL-0233, Procell, Wuhan, China) were cultured in RPMI1640 medium containing 10% FBS and 1% penicillin/streptomycin. .. Once the FTC238 cells reached 90% confluency, CD133+cells were isolated using immunomagnetic beads (130-097-049, Miltenyi Biotec, Bergisch Gladbach, Germany), obtaining THCA stem cells (FTC238-S). ..

    Article Title: TREM1 is essential for maintaining stemness of liver cancer stem-like cells in hepatocellular carcinoma
    Article Snippet: .. CD133 + cells were isolated using the CD133 Microbead Kit (130-097-049, Miltenyi Biotec). .. Briefly, cells were incubated with CD133 microbeads and then passed through an LS column (130-042-401, Miltenyi Biotec) placed in the magnetic field of a MACS separator (130-042-302, Miltenyi Biotec) attached to a MACS multistand (130-042-303, Miltenyi Biotec).

    Article Title: Method for manufacturing a patient specific cell population using a multifunctional self-contained apparatus and tubing set
    Article Snippet: .. For reducing of the final volume, three methods are possible: The final volume of isolated or enriched CD133+ cells can be reduced by 1. elution from the column in small volume, 2. filtration after magnetic separation, or 3. using the AutoMACS column (Miltenyi Biotec GmbH, Germany). ..

    Article Title: Effects of Notch signaling on proliferation, angiogenesis, and adipogenesis of hemangioma-derived stem cells
    Article Snippet: .. CD133 + cells were isolated from the single-cell suspension via magnetic-activated cell sorting (Miltenyi Biotec, Inc., Auburn, CA, USA) using anti-CD133 microbeads. .. Cells were cultured on fibronectin-coated plates (5 μg/cm2) in endothelial growth medium-2 (EGM-2; Lonza Group, Ltd., Basel, Switzerland) supplemented with 20% FBS and 1% penicillin/streptomycin (HyClone).

    Single Cell:

    Article Title: Effects of Notch signaling on proliferation, angiogenesis, and adipogenesis of hemangioma-derived stem cells.
    Article Snippet: .. CD133+ cells were isolated from the single-cell suspension via magnetic-activated cell sorting (Miltenyi Biotec, Inc., Auburn, CA, USA) using anti-CD133 microbeads. .. Cells were cultured on fibronectin-coated plates (5 μg/cm2) in endothelial growth medium-2 (EGM-2; Lonza Group, Ltd., Basel, Switzerland) supplemented with 20% FBS and 1% penicillin/streptomycin (HyClone).

    Article Title: Effects of Notch signaling on proliferation, angiogenesis, and adipogenesis of hemangioma-derived stem cells
    Article Snippet: .. CD133 + cells were isolated from the single-cell suspension via magnetic-activated cell sorting (Miltenyi Biotec, Inc., Auburn, CA, USA) using anti-CD133 microbeads. .. Cells were cultured on fibronectin-coated plates (5 μg/cm2) in endothelial growth medium-2 (EGM-2; Lonza Group, Ltd., Basel, Switzerland) supplemented with 20% FBS and 1% penicillin/streptomycin (HyClone).

    Suspension:

    Article Title: Effects of Notch signaling on proliferation, angiogenesis, and adipogenesis of hemangioma-derived stem cells.
    Article Snippet: .. CD133+ cells were isolated from the single-cell suspension via magnetic-activated cell sorting (Miltenyi Biotec, Inc., Auburn, CA, USA) using anti-CD133 microbeads. .. Cells were cultured on fibronectin-coated plates (5 μg/cm2) in endothelial growth medium-2 (EGM-2; Lonza Group, Ltd., Basel, Switzerland) supplemented with 20% FBS and 1% penicillin/streptomycin (HyClone).

    Article Title: Effects of Notch signaling on proliferation, angiogenesis, and adipogenesis of hemangioma-derived stem cells
    Article Snippet: .. CD133 + cells were isolated from the single-cell suspension via magnetic-activated cell sorting (Miltenyi Biotec, Inc., Auburn, CA, USA) using anti-CD133 microbeads. .. Cells were cultured on fibronectin-coated plates (5 μg/cm2) in endothelial growth medium-2 (EGM-2; Lonza Group, Ltd., Basel, Switzerland) supplemented with 20% FBS and 1% penicillin/streptomycin (HyClone).

    FACS:

    Article Title: Effects of Notch signaling on proliferation, angiogenesis, and adipogenesis of hemangioma-derived stem cells.
    Article Snippet: .. CD133+ cells were isolated from the single-cell suspension via magnetic-activated cell sorting (Miltenyi Biotec, Inc., Auburn, CA, USA) using anti-CD133 microbeads. .. Cells were cultured on fibronectin-coated plates (5 μg/cm2) in endothelial growth medium-2 (EGM-2; Lonza Group, Ltd., Basel, Switzerland) supplemented with 20% FBS and 1% penicillin/streptomycin (HyClone).

    Article Title: Effects of Notch signaling on proliferation, angiogenesis, and adipogenesis of hemangioma-derived stem cells
    Article Snippet: .. CD133 + cells were isolated from the single-cell suspension via magnetic-activated cell sorting (Miltenyi Biotec, Inc., Auburn, CA, USA) using anti-CD133 microbeads. .. Cells were cultured on fibronectin-coated plates (5 μg/cm2) in endothelial growth medium-2 (EGM-2; Lonza Group, Ltd., Basel, Switzerland) supplemented with 20% FBS and 1% penicillin/streptomycin (HyClone).

    Filtration:

    Article Title: Method for manufacturing a patient specific cell population using a multifunctional self-contained apparatus and tubing set
    Article Snippet: .. For reducing of the final volume, three methods are possible: The final volume of isolated or enriched CD133+ cells can be reduced by 1. elution from the column in small volume, 2. filtration after magnetic separation, or 3. using the AutoMACS column (Miltenyi Biotec GmbH, Germany). ..

    Incubation:

    Article Title: TREM1 is essential for maintaining stemness of liver cancer stem-like cells in hepatocellular carcinoma
    Article Snippet: .. After removing the column from magnetic field, the retained CD133 + cells were eluted and subsequently incubated with EpCAM microbeads (130-061-101, Miltenyi Biotec). ..

    Purification:

    Article Title: An HTLV-1-Infected Humanized Mouse Model Expressing HLA-A*02:01 Demonstrates Effective CTL-Mediated Suppression of HTLV-1
    Article Snippet: Mononuclear cells (MNCs) were isolated from umbilical cord blood by density gradient centrifugation using Lymphoprep (Serumwerk Bernburg, Bernburg, Germany), following the manufacturer’s instructions. .. CD133 + cells were subsequently purified from the MNCs using a CD133 MicroBead Kit (Miltenyi Biotec, Tokyo, Japan), as per the manufacturer’s protocol. ..



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    Knockdown of YWHAB promotes stemness and chemoresistance in OCPM. (A) The diameter and the number of the spheroids derived from YWHAB-knockdown and control primary CR OCPM cells were tested (one-way ANOVA; n = 3 biological replicates). (B) The mRNA level of <t>CD133</t> (left) and the ALDH activity (right) in indicated cells were analyzed by quantitative PCR and ALDH activity assay, respectively (one-way ANOVA; n = 3 biological replicates). (C) The proportions of CD133 + ALDH + cells in indicated cells were analyzed by flow cytometry (one-way ANOVA; n = 3 biological replicates). (D) The frequencies of in vitro sphere-forming cells (left) and in vivo tumor-initiating cells (right) in indicated cells were analyzed by limiting dilution assay (extreme limiting dilution analyses, for in vitro assay, n = 50 biological replicates per group, three groups (1, 5, and 10 cells per well); for in vivo assay, n = 10 biological replicates per group, three groups (8, 40, and 200 cells per mice)). (E) The IC 50 values of cisplatin in indicated primary CR OCPM cells were tested ( n = 3 biological replicates). ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001.
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    Image Search Results


    Knockdown of YWHAB promotes stemness and chemoresistance in OCPM. (A) The diameter and the number of the spheroids derived from YWHAB-knockdown and control primary CR OCPM cells were tested (one-way ANOVA; n = 3 biological replicates). (B) The mRNA level of CD133 (left) and the ALDH activity (right) in indicated cells were analyzed by quantitative PCR and ALDH activity assay, respectively (one-way ANOVA; n = 3 biological replicates). (C) The proportions of CD133 + ALDH + cells in indicated cells were analyzed by flow cytometry (one-way ANOVA; n = 3 biological replicates). (D) The frequencies of in vitro sphere-forming cells (left) and in vivo tumor-initiating cells (right) in indicated cells were analyzed by limiting dilution assay (extreme limiting dilution analyses, for in vitro assay, n = 50 biological replicates per group, three groups (1, 5, and 10 cells per well); for in vivo assay, n = 10 biological replicates per group, three groups (8, 40, and 200 cells per mice)). (E) The IC 50 values of cisplatin in indicated primary CR OCPM cells were tested ( n = 3 biological replicates). ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001.

    Journal: Genes & Diseases

    Article Title: Restriction of YWHAB-mediated YAP cytoplasmic retention is a novel mechanism underlying stemness maintenance and chemoresistance in ovarian cancer peritoneal metastasis

    doi: 10.1016/j.gendis.2025.101519

    Figure Lengend Snippet: Knockdown of YWHAB promotes stemness and chemoresistance in OCPM. (A) The diameter and the number of the spheroids derived from YWHAB-knockdown and control primary CR OCPM cells were tested (one-way ANOVA; n = 3 biological replicates). (B) The mRNA level of CD133 (left) and the ALDH activity (right) in indicated cells were analyzed by quantitative PCR and ALDH activity assay, respectively (one-way ANOVA; n = 3 biological replicates). (C) The proportions of CD133 + ALDH + cells in indicated cells were analyzed by flow cytometry (one-way ANOVA; n = 3 biological replicates). (D) The frequencies of in vitro sphere-forming cells (left) and in vivo tumor-initiating cells (right) in indicated cells were analyzed by limiting dilution assay (extreme limiting dilution analyses, for in vitro assay, n = 50 biological replicates per group, three groups (1, 5, and 10 cells per well); for in vivo assay, n = 10 biological replicates per group, three groups (8, 40, and 200 cells per mice)). (E) The IC 50 values of cisplatin in indicated primary CR OCPM cells were tested ( n = 3 biological replicates). ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001.

    Article Snippet: After the spheroid cells reached 60% confluence, CD133 + spheroid cells were separated by magnetic-assisted cell sorting technology (#130-100-857, Miltenyi Biotech) according to the manual.

    Techniques: Knockdown, Derivative Assay, Control, Activity Assay, Real-time Polymerase Chain Reaction, Flow Cytometry, In Vitro, In Vivo, Limiting Dilution Assay

    The effects of YWHAB knockdown on the stemness were significantly abolished in YAP5SA-overexpressing cells. (A) The diameter and the number of the spheroids derived from YAP5SA-overexpressing and empty-vector control cells transfected with shRNAs against YWHAB and control shRNAs were measured (one-way ANOVA; n = 3 biological replicates). (B) The mRNA level of CD133 (left) and the ALDH activity (right) in indicated cells were determined by quantitative PCR and ALDH activity assay, respectively (one-way ANOVA; n = 3 biological replicates). (C) The proportion of CD133 + ALDH + cells in indicated cells was analyzed by flow cytometry (one-way ANOVA; n = 3 biological replicates). (D) The frequencies of in vitro sphere-forming cells (left) and in vivo tumor-initiating cells (right) in indicated cells were analyzed by limiting dilution assay (extreme limiting dilution analyses; for in vitro assay, n = 50 biological replicates per group, three groups (1, 5, and 10 cells per well); for in vivo assay, n = 10 biological replicates per group, three groups (8, 40, and 200 cells per mice)). (E) The IC 50 values of cisplatin in indicated cells were tested by cell viability assay ( n = 3 biological replicates). The inhibition rate of 100 uM cisplatin in indicated cells was provided. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001.

    Journal: Genes & Diseases

    Article Title: Restriction of YWHAB-mediated YAP cytoplasmic retention is a novel mechanism underlying stemness maintenance and chemoresistance in ovarian cancer peritoneal metastasis

    doi: 10.1016/j.gendis.2025.101519

    Figure Lengend Snippet: The effects of YWHAB knockdown on the stemness were significantly abolished in YAP5SA-overexpressing cells. (A) The diameter and the number of the spheroids derived from YAP5SA-overexpressing and empty-vector control cells transfected with shRNAs against YWHAB and control shRNAs were measured (one-way ANOVA; n = 3 biological replicates). (B) The mRNA level of CD133 (left) and the ALDH activity (right) in indicated cells were determined by quantitative PCR and ALDH activity assay, respectively (one-way ANOVA; n = 3 biological replicates). (C) The proportion of CD133 + ALDH + cells in indicated cells was analyzed by flow cytometry (one-way ANOVA; n = 3 biological replicates). (D) The frequencies of in vitro sphere-forming cells (left) and in vivo tumor-initiating cells (right) in indicated cells were analyzed by limiting dilution assay (extreme limiting dilution analyses; for in vitro assay, n = 50 biological replicates per group, three groups (1, 5, and 10 cells per well); for in vivo assay, n = 10 biological replicates per group, three groups (8, 40, and 200 cells per mice)). (E) The IC 50 values of cisplatin in indicated cells were tested by cell viability assay ( n = 3 biological replicates). The inhibition rate of 100 uM cisplatin in indicated cells was provided. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001.

    Article Snippet: After the spheroid cells reached 60% confluence, CD133 + spheroid cells were separated by magnetic-assisted cell sorting technology (#130-100-857, Miltenyi Biotech) according to the manual.

    Techniques: Knockdown, Derivative Assay, Plasmid Preparation, Control, Transfection, Activity Assay, Real-time Polymerase Chain Reaction, Flow Cytometry, In Vitro, In Vivo, Limiting Dilution Assay, Viability Assay, Inhibition